Vectors based on lentivirus backbones have revolutionized our ability to transfer genesinto many cell types. Lentiviral vectors integrate into the chromatin of target cells and do not transfer any viral genes causing vector replication. Both of these features arecommonly used in Gene therapy and have been used clinically in individuals sufferingfrom cancer, infections and genetic diseases. It has been discovered that T-cells can be genetically modified to be used as effective weapons against cancer: therefore virus mustbe produced to deliver the gene of interest into the T-cells. In this project, lentiviralvectors have been produced to transfer the gene coding for a chimeric antigen receptor(CAR) which is directed to CD19 on B-cells.

The WT1 gene is a complex gene originally known to suppress cancer in kidneys. Studies of WT1 knockout mice have confirmed the important role of WT1 in the pathogenesis of Wilms? tumour, a tumour which counts for 95% of all childhood renal tumours. In that case the WT1 gene acts as a tumour suppressor gene. Subsequent research has shown that the WT1 gene in many other cases acts as an oncogene, e g in leukemia or lung cancer (even though these cancer forms can emerge as a result of many other aetiological factors).

The RECK gene is a relatively new discovered gene with important implications for cancer research. The research has been primarily concentrated on the human gene with the ultimate aim to identify the invasive characteristics. Up regulated RECK is linked to significantly prolonged survival rates in patients with severe forms of malignancies. RECK is normally expressed in all cells of the body and has an important role in the balance between destructive and constructive features of the extracellular matrix. The RECK protein is a membrane-bound glycoprotein that inhibit matrix metalloproteinases which has the function of breaking down the ECM. There is a significant correlation between RECK gene expression and the formation of new vessels, presumably via the mediation of VEGF which is an important and powerful inducer of angiogenesis.

The increased frequency of reported Methicillin-resistant Staphylococcus pseudintermedius (MRSP) is of great concern to small animal healthcare due to limited options of antimicrobial therapy. The aim of this literature study is to investigate 1) characteristics and classification of S. pseudintermedius, 2) resistance mechanisms and emergence of MRSP, 3) risk factors for selection of MRSP, and 4) clonal distribution around the world. S. pseudintermedius is a skin and mucosal commensal in healthy dogs and cats, but can cause infections, including pyoderma. Beta-lactam antibiotics inhibit bacterial growth by binding to the penicillin binding protein (PBP), a vital enzyme in the synthesis of the bacterial cell wall.

At two nursing homes for people with dementia a qualitative study was performed. The purpose was to investigate the therapy dog?s meaning to people with dementia from their perspective. Two women and five men with dementia were interviewed, everyone being somewhere between 60 and 87 years of age. All but one had previously owned a dog at some point in their lives.

Alternative splicing is a process that partly rejects the common definition of a gene ? that one gene codes for one specific protein. By variable combination of coding regions (exons) and exclusion of non-coding regions (introns), formation of several different mRNA-transcripts, and consequently several different proteins, can derive from the same gene. Alternative splicing is an important condition for the development of complex life forms, but it is also a highly sensitive process and inaccurate splicing is the cause of approximately 15 % of mutations that cause genetic diseases. This article presents four genes, BRCA1, BRCA2, ER? and ER?, and inaccurate splicing of these genes increases the risk of developing cancer, particularly breast cancer and ovarian cancer.

Various Fusarium species causes some of the most common cereal diseases worldwide. Besides the yield losses that can be a result of these diseases, strains from several Fusarium species can produce mycotoxins, some very toxic. The aim of this study was to investigate which Fusarium species and how many that occurred in winter wheat grains from Mälardalen and Kalmar län, if there was any difference in the distribution of Fusarium fungi between the regions and the potential within species to produce mycotoxins belonging to the group trichothecenes. Winter wheat grains collected in 2009 from ten fields (unsprayed plots) in the two regions were analysed for Fusarium species. PCR was used to amplify the TEF region where after the samples were sequenced.

Today therapy dogs (Canis lupus familiaris) service people more than ever and different categories of work places use therapy dogs to help their caretakers. Many studies have been made on the positive effects in patients when they are given contact with a dog, but very little have been done on how the dog experience its situation and what kind of behaviour a therapy dog shows during its working days. This work was made to investigate the behaviours that educated therapy dogs in Sweden showed when they were in contact with a caretaker and to find out if the dogs showed any signs of stress or some other kind of discomfort. To do this a survey was sent out to 17 educated therapy dog handlers in Sweden that together had 18 educated therapy dogs. The survey contained 39 questions about the daily work of the therapy dogs when they were together with caretakers and some questions about the therapy dogs spare time. A question about what kind of dog breed the therapy dog handlers had were also in the survey to investigate if some breeds were more usual as therapy dogs.

The aim of the study was to illustrate how music therapy can be used in the care of patients with schizophrenia and other psychotic illness.The method used was a literature review. Scientific articles were searched in PubMed, Cinahl, PsycINFO och Scopus databases with an outcome of ten articles, with in total 330 participants, that underwent quality and outcomes analysis.The results showed that music therapy could provide potential improvement in the patients' social functions and that it could also improve negative, some positive and general schizophrenia and psychotic symptoms in some patients. The form of music therapy used was often creative music activities with music therapist, with or without structure, or individually customized. The length of treatment varied widely. It was not possible to draw conclusions about which type of music therapy was best.

Staphylococcus aureus belongs to the normal flora. Many healthy people are colonized by the bacterium mainly in the nose but also on the skin and on other mucous membranes without showing symptoms. After damage to the skin, the bacterium can enter the wound and cause infections. Methicillin-resistant S. aureus (MRSA) is resistant to b-lactam antibiotics such as penicillin and methicillin.

This review article presents the splicing process during messenger RNA maturation and how it is regulated by different Cis-regulatory RNA-sequence elements and splicing factors. A more detailed description of the process alternative splicing and its importance to the function of genes from the model organism Arabidopsis thaliana is also given. A single eukaryotic gene can by the process alternative splicing (AS) give rise to a number of functionally mature mRNA-molecules, which in turn encodes for structurally and/or functionally different proteins. During the course of evolution, the process alternative splicing has thus shown to be effective in increasing transcriptome and proteome diversity of most eukaryotic organisms. This suggests therefore that the dominant theory in molecular biology, a gene encodes for a protein, needs to be corrected.

Mast cells have for a long time been known to accumulate around tumors (Maltby et al., 2009). Studies show that they may be important and sometimes essential in tumor angiogenesis, tumor growth and metastasis of tumors (Soucek, et al., 2007; Xiang et al., 2010). In an expression array study (Wensman et al, submitted manuscript) performed on mouse mast cells exposed to tumor conditioned medium, the gene 4-1BB was among the most up-regulated genes compared to control medium. 4-1BB codes for a membrane receptor protein of the tumor necrosis factor superfamily and has been shown to be important in T cell regulation in tumor diseases. This study was performed to investigate if human mast cells up-regulate 4-1BB when they get exposed to tumor conditioned medium.

The object of this thesis is the foundation of ethics. The question is whether there exists a universal core to ethics consisting of a fundamental ethical principle across cultures. This principle could for example be the so-called Golden Rule, which goes as follows: ?You should do to others what you want them to do to you?. The Golden Rule is to be found in many of the world?s religions and is also reflected in secular society.

Animal-assisted therapy had its large breakthrough in the 1960-ties when a psychiatrist named Boris Levinson discovered the great advantages of involving animals in the treatment of patients. Therapy with animals involves that animals are used in a systematic way, in a person's process of treatment. The most distinguished companion animal used within this area is the dog, but horses, cats, rabbits, birds, dolphins and the most common farm animals are used in animal-assisted therapy. Most animals that are to be used for this specific purpose need to be trained in order to maintain their physical and mental health, but also to minimize the risks for the people that are involved. Most studies within this research area are focused on the positive effects on the patients and less on the effects on the animals.

Historically, the horse has in many ways been important to human beings. Today, Equine Assisted Therapy is a form of treatment in which the horse is used as a tool from the treatment of the client. Supported Equine therapy has a therapeutic purpose and may vary depending on the patient's needs and the professionals work competence. Various studies show that animals have a positive impact on people. The aim of our study was to explore how Equine Assisted Therapy is used within the practical fields of social work in Sweden.